The in-vitro serum testing for IgE antibodies developed against potential allergens put into the blood serum is a component of the allergy animal panel confirmatory test. It hurts a lot to test for allergies using the conventional method, which involves scraping the skin after allergens have been applied. The allergens typically employed for the test are those from the regularly domesticated animals living close to people, such as cat epithelium and dander, dog dander, cow dander, and horse dander. In the real world, people with asthma and others experience severe allergic reactions from these allergens, including itchiness, skin rashes, watery eyes, allergic rhinitis, and severe breathing difficulties. Since some allergies are not IgE mediated, alternative test procedures may be used to identify them.