The diphtheria culture test is used to find out if the bacteria Corynebacterium diphtheria are present. When diphtheria is thought to be present, this test is used. The respiratory tract is affected by diphtheria, a dangerous and frequently fatal bacterial infection. The skin, nose, and throat may all harbour these bacteria. All individuals with suspected cases, as well as their close contacts, have clinical specimens for culture obtained from their nose, nasopharynx, and throat. Additionally, swabs from below the membrane are obtained, or a portion of the membrane is cut away. As soon as diphtheria is suspected, specimens are collected for cultivation.