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A partial thromboplastin time (PTT) test measures the time it taken for a blood clot to form. Normally, when you get a cut or injury that causes bleeding, proteins in your blood called coagulation factors work together to form a blood clot. The clot stops you from losing too much blood. Mixing studies help distinguish clotting time prolongation due to a coagulation factor deficiency or an inhibitor, e.g. lupus anticoagulant. To perform a mixing study, mix patient plasma and normal pooled plasma and measure the clotting time that was initially prolonged. If the clotting time corrects, this suggests a factor deficiency; if the clotting time does not correct, this suggests presence of a circulating inhibitor. Mixing study may direct further coagulation testing but it is not by itself diagnostic, e.g. may aid in selecting factor assays
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